Protocols

Primary Microglial Cultures From Mouse Cerebral Cortex

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PI: Josep Saura
Affiliation: University of Barcelona
Submitted by: Josep Saura
Posted Date: 07 Sep 2010

Brief Description

This protocol describes the preparation of primary cultures consisting of >95% microglia from neonatal mouse cerebral cortex. It also works for late embryos, for rat and for most CNS regions. Our protocols for preparing mixed glial or highly enriched astroglial cultures are also available in this database.

References

  1. . High-yield isolation of murine microglia by mild trypsinization. Glia. 2003 Dec;44(3):183-9. PubMed.

Keywords

microglia, mouse, rat, rodent, primary cell, glia

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