Protocols

Primary Astroglial Cultures From Mouse Cerebral Cortex

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PI: Josep Saura
Affiliation: University of Barcelona
Submitted by: Josep Saura
Posted Date: 07 Sep 2010

Brief Description

This protocol describes how to prepare primary cultures consisting of >95% astrocytes from neonatal mouse cerebral cortex. It also works for late embryos, for rat and for most CNS regions. Protocols for preparing mixed glial or highly enriched astroglial cultures are also available in this database.

References

  1. . Microglial cells in astroglial cultures: a cautionary note. J Neuroinflammation. 2007;4:26. PubMed.

Keywords

astrocyte, primary cells, microglia, Ara-C

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