This protocol describes how to prepare primary cultures consisting of >95% astrocytes from neonatal mouse cerebral cortex. It also works for late embryos, for rat and for most CNS regions. Protocols for preparing mixed glial or highly enriched astroglial cultures are also available in this database.
- Saura J. Microglial cells in astroglial cultures: a cautionary note. J Neuroinflammation. 2007 Oct 15;4:26. PubMed.
astrocyte, primary cells, microglia, Ara-C