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Immunohistochemistry for Brain Sections (ABC and Immunofluorescence)

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PI: Ralph Nixon
Affiliation: New York University School of Medicine and the Nathan Kline Institute
Submitted by: Ralph Nixon
Posted Date: 19 Jun 2009

Brief Description

Frequently used on human and mouse brain sections for detecting pathologies in the endocytic-autophagic-lysosomal pathways such as changes in the numbers, types and distribution of vacuoles, and expression levels of individual molecules in the pathways.

References

  1. . Lysosomal proteinase antigens are prominently localized within senile plaques of Alzheimer's disease: evidence for a neuronal origin. Brain Res. 1990 Apr 16;513(2):181-92. PubMed.
  2. . Presenilin mutations in familial Alzheimer disease and transgenic mouse models accelerate neuronal lysosomal pathology. J Neuropathol Exp Neurol. 2004 Aug;63(8):821-30. PubMed.
  3. . Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577-80. PubMed.
  4. . Immunocytochemistry of formalin-fixed human brain tissues: microwave irradiation of free-floating sections. Brain Res Brain Res Protoc. 1998 Jan;2(2):109-19. PubMed.
  5. . Monitoring autophagy in Alzheimer's disease and related neurodegenerative diseases. Methods Enzymol. 2009;453:111-44. PubMed.

Keywords

avidin-biotin complex, ABC, immunofluorescence, brain sections, confocal microscopy

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