Greber S, Lubec G, Cairns N, Fountoulakis M.
Decreased levels of synaptosomal associated protein 25 in the brain of patients with Down syndrome and Alzheimer's disease.
Electrophoresis. 1999 Apr-May;20(4-5):928-34.
PubMed.
In this paper the authors used 2D gels to determine levels of SNAP-25 and GFAP. Levels of SNAP-25 were found to decrease in AD and DS, while GFAP was increased. The authors state 'Decreased snap-25 protein levels in the brain of DS and AD may reflect impaired synaptogenesis or represent neuronal loss. Findings of increased GFAP, a marker for neuronal loss, along with data from literature would support the notion of decreased snap-25 secondary to neuronal decay.' The use of GFAP as a marker of neuronal loss is quite a stretch, which leaves unresolved the issue of SNAP-25 decrease vs. neuronal loss (is that what 'decay' refers to?). The abstract does not make it clear whether or not the identification of SNAP-25 and GFAP in their 2D gels was verified with antibodies.
Comments
Banner Research Institute
In this paper the authors used 2D gels to determine levels of SNAP-25 and GFAP. Levels of SNAP-25 were found to decrease in AD and DS, while GFAP was increased. The authors state 'Decreased snap-25 protein levels in the brain of DS and AD may reflect impaired synaptogenesis or represent neuronal loss. Findings of increased GFAP, a marker for neuronal loss, along with data from literature would support the notion of decreased snap-25 secondary to neuronal decay.' The use of GFAP as a marker of neuronal loss is quite a stretch, which leaves unresolved the issue of SNAP-25 decrease vs. neuronal loss (is that what 'decay' refers to?). The abstract does not make it clear whether or not the identification of SNAP-25 and GFAP in their 2D gels was verified with antibodies.
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