Blum G, von Degenfeld G, Merchant MJ, Blau HM, Bogyo M.
Noninvasive optical imaging of cysteine protease activity using fluorescently quenched activity-based probes.Nature Chemical Biology.
2007 Sep 9;
REAGENTS/MATERIAL: Antibodies used in this study are, fot immunoprecipitation: anti-mouse cathepsin B (provided by E Weber, Halle, Germany) and rat monoclonal anti-mouse cathepsin L (R&D Systems)
We have generated a series of quenched near-infrared fluorescent activity-based probes (qNIRF-ABPs) that covalently target the papain-family cysteine proteases shown previously to be important in multiple stages of tumorigenesis. These 'smart' probes emit a fluorescent signal only after covalently modifying a specific protease target. After intravenous injection of NIRF-ABPs into mice bearing grafted tumors, noninvasive, whole-body imaging allowed direct monitoring of cathepsin activity. Importantly, the permanent nature of the probes also allowed secondary, ex vivo biochemical profiling to identify specific proteases and to correlate their activity with whole-body images. Finally, we demonstrate that these probes can be used to monitor small-molecule inhibition of protease targets both biochemically and by direct imaging methods. Thus, NIRF-ABPs are (i) potentially valuable new imaging agents for disease diagnosis and (ii) powerful tools for preclinical and clinical testing of small-molecule therapeutic agents in vivo.