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REAGENTS/MATERIAL: Sandwich ELISA for Ab and ApoE:
For Ab ELISA, HJ2 (anti-Ab35-40) and HJ7.4 (anti-Ab37-42) were used as capture
antibodies and HJ5.1-biotin (anti-Ab13-28) as the detection antibody. Monoclonal anti-human-ApoE
(WUE4) and anti-ApoE (Calbiochem) were used for apoE ELISA.
For in vivo microdialysis experiments, human
Ab1-x from collected fractions was measured using m266 antibody (anti-Ab13-
28) to capture and 3D6-biotinylated antibody (anti-Ab1-5) to detect. Quantitative Analyses of Neuroinflammatory Response
Brain sections cut with a freezing sliding microtome were immunostained with
anti-CD11b antibody (BD Pharmingen),
anti-CD45 (Serotec), and
anti-GFAP (Chemicon). Western Blot
Blots were probed for LDLR with antibodies from Novus,
and a gift from Dr. Guojun Bu at Washington University.
Blots were also probed for CT22 (Zymed),
and ApoJ (Covance).