The peptides used (Ab1-40, Ab 1-42, Ab1-30-NH2, and K6Ab1-30-NH2) were synthesized at the Keck Foundation (Yale University, New Haven, CT), The peptide used for the immunizations, K6Ab1-30-NH2, maintains the two major immunogenic sites of Abpeptides, which are residues 1 to 11 and 22 to 28 of Ab 1-42 based on the antigenic index of Jameson and Wolf, and on preliminary results obtained in our laboratory.
Immunostaining on mouse brains used 6E10, 1:1000 (gift of Richard Kascsak, Institute for Basic Research), Anti-interleukin-1b,1:250 (Endogen, Rockford, IL), anti-OX-42, 1:250 (Biosource Int.), anti-GFAP, 1:500 (DAKO). For double-labeling of IL-1b and Amyloidb plaques, sections were first stained for IL-1b, (DAB/Ni; black) in which peroxidase was the enzyme. Sandwich ELISA was performed with anti-Ab R162 specific for Ab 40 and anti-Ab R165 specific for Ab 42.