Adlard PA, Cherny RA, Finkelstein DI, Gautier E, Robb E, Cortes M, Volitakis I, Liu X, Smith JP, Perez K, Laughton K, Li QX, Charman SA, Nicolazzo JA, Wilkins S, Deleva K, Lynch T, Kok G, Ritchie CW, Tanzi RE, Cappai R, Masters CL, Barnham KJ, Bush AI. Rapid restoration of cognition in Alzheimer's transgenic mice with 8-hydroxy quinoline analogs is associated with decreased interstitial Abeta. Neuron. 2008 Jul 10;59(1):43-55. PubMed Abstract

REAGENTS/MATERIAL:
Western blot was utilized to assess total tau with rabbit anti-human total tau (DAKO), and tau phosphorylated at Ser396 with rabbit anti-tau phos Ser396 (Biosource/Invitrogen). We examined brain synaptophysin levels by western blot using anti-synaptophysin (Chemicon). The amount of dityrosine crosslinked Aβ (Barnham et al., 2004) was assayed using mouse monoclonal anti-dityrosine (1C3) (Dr. Yoji Kato, Himeji Institute of Technology, Himeji, Japan), We assesed soluble oligomeric Aβ by western blots probed with rabbit oligomer-specific anti-Aβ A11 (Kayed et al., 2003) (gift from Dr. Charles Glabe, University of California at Irvine, USA), APP by western blots using mouse monoclonal anti-APP (22C11) (Chemicon), and total Aβ by western blots using mouse monoclonal anti-Aβ (WO2), 6E10 and 4G8