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REAGENTS/MATERIAL: Phospho-specific a-pS1328 SPAR antibodies were generated by immunizing rabbits (Covance Research Products) with a SPAR peptide corresponding to
residues 1323–1335, in which S1328 was phosphorylated. Phospho-antibodies were purified first by negative selection over a column of covalently
attached unphosphorylated peptide, followed by binding to a column of phosphorylated peptide. Polyclonal GKBD-SPAR antibodies
were generated by immunizing rabbits with purified MBP-GKBD fusion protein.
The polyclonal C-SPAR antibody has been described in Pak et al., 2001.
Mouse monoclonal PSD-95 antibody K28/43 was a gift from J. Trimmer (UC Davis);
rabbit polyclonal p25/p35 antibody was a gift from Li-Huei Tsai (MIT).
The following commercial antibodies were used: mouse monoclonal anti-myc, agarose conjugate (9E10) (Santa Cruz); rabbit anti-GST (Z-5) (Santa Cruz Biotechnology);
mouse monoclonal anti-α-tubulin (B-5-1-2) (Sigma Aldrich);
rabbit anti-GFP (MBL International)