Anti-NgR (AF1440) antibody was from R & D Systems, the 369 anti-C-terminal-APP antibody was a generous gift from S. S. Sisodia (University of Chicago, Chicago, IL). The anti-N-terminal-APP 5228 antibody, the anti-Ab1–17 6E10 antibody, and the anti-C-terminal-APP 5352 antibody were obtained from Chemicon (Temecula, CA). The anti-Ab 4G8 antibody and the anti-C-terminal-APP 51–2700 antibodies were from Signet Laboratories (Dedham, MA) and Zymed Laboratories (South San Francisco, CA), respectively. The anti-secretet APP (SAPP)swe-specific 6A1 antibody was from Immunobiological Laboratories (Gunma, Japan).
Transgenic APPswe/PSEN-1(dE9) mice were from The Jackson Laboratory (Bar Harbor, ME) (stock #04462).
Ab ELISA assays were performed according to the instructions of the manufacturer (Biosource, Camarillo, CA). Neuritic dystrophy was visualized by staining with monoclonal-anti-synaptophysin antibodies (Sigma, St. Louis, MO) in parasagittal paraffin-embedded sections.