Generated mice with targeted alleles for FE65 and FE65L1. Viable FE65-/-; FE65L1-/- mice are smaller than their littermates and often displayed bilateral circling.
The pan-FE65 antibody generated against a glutathione S-transferase-FE65L1 fusion protein was previously described (Chang et al, 2003). The Tuj1 (Covance), anti-NeuN (Chemicon Int.), GFAP (Sigma-Aldrich) and CD-45 (Serotec) antibodies were used to stain immature neurons, postmitotic neuronal nuclei, glia and microglia, respectively. Antibodies directed against EHS-laminin and chondroitin sulfate modifications (both from Sigma-Aldrich) were used to detect extracellular matrix (ECM) proteins and reelin antibodies (G10, a gift from A Goffinet, University of Louvain, Belgium) detected secreted reelin and reelin-positive cells.APP antibodies included APP 22C11 (Chemicon Int.) and A8717 (Sigma-Aldrich). Anti-TAG-1 (Developmental Studies Hybridoma Bank) and anti-L1 antibodies (Chemicon Int.) were used to stain corticofugal and thalamocortical neurons, respectively.