Amyloid precursor protein promotes post-developmental neurite arborization in the Drosophila brain. - AlzForum Alzheimer Research Paper of the Week


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Home: Papers of the Week

Annotation

	Leyssen M, Ayaz D, Hébert SS, Reeve S, De Strooper B, Hassan BA. Amyloid precursor protein promotes post-developmental neurite arborization in the Drosophila brain. EMBO J. 2005 Aug 17;24(16):2944-55. PubMed Abstract

	Comments on Paper and Primary News

	Comment by: Andre Delacourte
	Submitted 9 August 2005 \| Permalink	Posted 11 August 2005
	I recommend this paper

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REAGENTS/MATERIAL:
Immunohistochemistry:The APPL antibody (used in a dilution of 1:10 000) was obtained from K White, the PDF antibody (1:1000) from P Taghert, the Elav (1:500) antibody from the Developmental Studies Hybridoma Bank (University of Iowa). For Myc staining the polyclonal antibody (Upstate), for GFP staining rabbit anti-GFP (Molecular Probes) (1:1000), and for APP the 4G8 antibody (1:100) from Abcam or the B63.5 antibody (1:8000) were used. For actin labelling, brains were incubated with 1:1000 TRITC-Phalloidin (SIGMA) in PBT for 2 h at room temperature.