Mice used were PDAPP (Games, 1995) and mLRP2 (Borchelt, 1996). Double TG mice overexpressing APPV717F and mLRP2 transgenes were obtained by breeding homozygous (+/+) PDAPP mice with heterozygous (+/–) mLRP2 mice that were bred back into the C57BL/6 background for at least five generations.
Brain lysates, pellets and cerebrospinal fluid were used for measurement of soluble/insoluble Ab by sandwich ELISA for human Ab40 (2G3 antibody) and human Ab42 (21F12
antibody). For IHC, Brain sections were stained with a polyclonal antibody to HA epitope (Upstate Biotechnology) to detect mLRP2 or with a polyclonal antibody to full-length LRP to detect both endogenous LRP and mLRP2. A rabbit polyclonal pan-Ab antibody (BioSource International), followed by diaminobenzidine detection, was used to visualize Ab load.
Western blots were probed by 6E10 anti-Ab monoclonal antibody (Signet).