Immunoblots were probed with 1:3000 dilutions of rabbit anti-GR polyclonal antibodies (Santa Cruz Biotechnology). The blot was developed with 1:10,000 dilution of anti-rabbit secondary antibody coupled to HRP. For the ribosomal protein Tcm1P, the blot was incubated with a 1:1000 dilution of monoclonal Anti-Tcm1p antibody (gift of J. Warner) and developed with HRP coupled anti-mouse secondary antibody. For the proteinase assay for CPEBQ-GR526 a membrane was blotted with 1:2000 dilutions of rabbit anti-rat GR antibodies (Santa Cruz Biotechnology). For GR Western 1:200 dilution of GR monoclonal antibody was used.