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Home: Papers of the Week
Annotation


Hafezparast M, Klocke R, Ruhrberg C, Marquardt A, Ahmad-Annuar A, Bowen S, Lalli G, Witherden AS, Hummerich H, Nicholson S, Morgan PJ, Oozageer R, Priestley JV, Averill S, King VR, Ball S, Peters J, Toda T, Yamamoto A, Hiraoka Y, Augustin M, Korthaus D, Wattler S, Wabnitz P, Dickneite C, Lampel S, Boehme F, Peraus G, Popp A, Rudelius M, Schlegel J, Fuchs H, Hrabe de Angelis M, Schiavo G, Shima DT, Russ AP, Stumm G, Martin JE, Fisher EM. Mutations in dynein link motor neuron degeneration to defects in retrograde transport. Science. 2003 May 2;300(5620):808-12. PubMed Abstract

  
Comments on Paper and Primary News
  Comment by:  Takaomi Saido, ARF Advisor
Submitted 12 June 2003  |  Permalink Posted 13 June 2003
  I recommend this paper

This work further confirms the importance of axonal retrograde transport in the development of neurodegeneration (at least in motor neuron degeneration). Although whether the concept applies to Alzheimer research remains to be examined, it seems worth trying.

View all comments by Takaomi Saido
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REAGENTS/MATERIAL:

Antibodies used in this study: R325 (sc-9115, Santa Cruz) for cytoplasmic dynein heavy chain detection in WB, IHC, IP; MAB1618 (Chemicon) for cytoplasmic dynein intermediate chain in WB, IHC, IP; D5167, Clone 70.1, Sigma) for cytoplasmic dynein intermediate chain in IHC; NCL-SOD1 (Nococastra) for superoxice dismutase 1 (CuZn) (Clone SD-G6, S2147, Sigma) in IHC; Z334 (DAKO) for GFAP in IHC; Clone 2F11 (DAKO) for neurofilament in whole mount in situ hybridization; AB1981 (Chemicon) for neurofilament in IHC; Mab377 (Chemicon) for NeuN in IHC; Z0458 (DAKO) for CDK5 in IHC; TUJ1 (BabCo) for Beta III tubulin in whole mount in situ hybridization; CA1137 (affiniti) for CGRP in nerve ligiation; Asy3 (Biometra) for Synaptophysin in nerve ligiation; A1582 (Chemicon) for choline acetytransferase in nerve ligation.

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