Breeding pairs of HD-N171-82Q mice were kindly provided by D. R. Borchelt (Johns Hopkins University, Baltimore). These transgenic mice were mated continuously to hybrid (C3H/HEJxC57BL/6J F1; Taconic Farms) mice, and lines were maintained on the hybrid background. HD-N171-82Q (+/-, HD) mice and nontransgenic mice were used in this study.
For immunoblot and immunostaining methods the primary antibodies were mouse monoclonal anti-HSP-70, 1:5,000, Sigma; mouse monoclonal anti-caspase-1, 1:1,000, PharMingen; and mouse monoclonal anti-beta-actin, (1:5,000, Sigma). For immunostaining of mutant human N-terminal fragment of huntingtin, brain sections were incubated with mouse monoclonal antibody EM48 (1:500) as primary antibody.