Oosthuyse B, Moons L, Storkebaum E, Beck H, Nuyens D, Brusselmans K, Van Dorpe J, Hellings P, Gorselink M, Heymans S, Theilmeier G, Dewerchin M, Laudenbach V, Vermylen P, Raat H, Acker T, Vleminckx V, Van Den Bosch L, Cashman N, Fujisawa H, Drost MR, Sciot R, Bruyninckx F, Hicklin DJ, Ince C, Gressens P, Lupu F, Plate KH, Robberecht W, Herbert JM, Collen D, Carmeliet P. Deletion of the hypoxia-response element in the vascular endothelial growth factor promoter causes motor neuron degeneration. Nat Genet. 2001 Jun;28(2):131-8. PubMed Abstract

REAGENTS/MATERIAL:

"Knock-in" mice were generated by deleting the hypoxia-response element sequence in the Vegf promoter.

Immunohistochemistry used the following antibodies:glucose transporter-1 (sc-1605), Vegf (sc-152, Santa Cruz), Kdr (gift of H. Kataoka), Nrp, Chat (AB143, Chemicon), NF and NFp (Sterberger Monoclonals), GFAP (Z0334), ubiquitin (Z0458) from Dako and pimonidazole hydrochloride (Natural Pharmacia Int).