Lucas JJ, Hernández F, Gómez-Ramos P, Morán MA, Hen R, Avila J.
Decreased nuclear beta-catenin, tau hyperphosphorylation and neurodegeneration in GSK-3beta conditional transgenic mice.EMBO J.
2001 Jan 15;20(1-2):27-39.
PubMed Abstract, View on AlzSWAN
REAGENTS/MATERIAL: 8.0 kb AseI BitetO fragment was microinjected into single-cell CBAxC57BL/6 embryos for the generation of transgenic mice
7.51 antibody (Dr. Wischik)
PHF-1 antibody (Dr. Davies), react with tau when serines 396 and 404 are phosphorylated
12E8 antibody (Dr. Seubert), react with tau when serine 262 is phosphorylated
AD2 antibody (Dr. Mourton-Giles), react with tau when serines 396 and 404 are phosphorylated
OX42 antibody (Dr. Bovolenta)
USsnRNP antibody (Dr. Ortin)
GSK3beta and Beta-catenin antibodies (Transduction Laboratories)
Beta-tubulin antibody (Sigma)
GFAP antibody (PharMingen)
ED1 antibody (Serotec)
Cleaved caspase-3 antibody (Cell Signaling Technology)
FUTURE DIRECTION: More study on beta-catenin is needed, such as the target genes that are transactivated by beta-catenin that are responsible for the increased susceptibility of apoptosis. Further elucidation on the contribution of GSK-3beta to neurodegeneration.