Akiyama H, Kondo H, Ikeda K, Kato M, McGeer PL. Immunohistochemical localization of neprilysin in the human cerebral cortex: inverse association with vulnerability to amyloid beta-protein (Abeta) deposition. Brain Res. 2001 Jun 1;902(2):277-81. PubMed Abstract

REAGENTS/MATERIAL:
Brains from 15 patients with AD, three with diffuse Lewy body disease (DLBD) with AD pathology, two DLBD without AD pathology, two tangle only dementia, one schizophrenic, and three control subjects. Mouse monoclonal antibody SN5c/L4-1A1 against neprilysin, IgG, (Ancell) Mouse monoclonal antibody MEM78 against neprilysin, IgG, (Southern Biotechnol) Mouse monoclonal antibody FR4D11 against neprilysin, IgG, (NeoMarkers) Rabbit polyclonal antibody R37 raised against syntehtic peptide corresponding to residues 681-695 of APP695, obtained from Dr. Tsuyoshi Ishii (Sagamidai Hospital)

FUTURE DIRECTION:
More sensitive and quantitative methods needed to further investigate the relationship of histochemical localization of neprilysin and Abdeposition in brain, esp. in brain tissues with heavy Ab burden.