Researchers from Bart de Strooper's lab at the Flanders Interuniversity Institute for Biotechnology, reported in the 20 November Neuron that telencephalin (TLN), a member of the intercellular adhesion molecule (ICAM) family, binds to presenilins 1 and 2 (PS1 and PS2), components of the amyloid precursor protein (AβPP) γ-secretase complex that is implicated in the etiology of Alzheimer's disease.

TLN was identified from a yeast two-hybrid screen of a mouse hippocampus library using a C-terminal fragment of PS1 as bait. The group subsequently separated TLN from brain extracts using an immobilized PS1 C-terminal fragment. Surprisingly, the reverse experiment, using an immobilized fragment of the TLN transmembrane domain, separated both C-terminal and N-terminal fragments of PS1, indicating that both ends of the protein bind to TLN. PS1's TLN-binding sites are amino acids 1-163, which cover the first transmembrane domain, and the 39 C-terminal residues, which are predicted to lie in the cytosol. These findings suggest that, in vivo, the PS1 molecule may form a ring structure, with the C- and N-terminals coming into contact with TLN. In addition, the same PS1 sites were found to bind to AβPP, suggesting a common mode of interaction between presenilins and their molecular partners.

"Though telencephalin, which is localized in the telencephalon and is not a PS substrate, is not directly involved in Alzheimer's disease, this data may be functionally important in understanding how presenilins work," commented Christian Haass, Ludwig-Maximilians University, Munich. "The ring structure is almost certainly correct, though proving this directly in vivo will be difficult."-Tom Fagan.
References: Annaert WG, Esselens C, Baert V, Boeve C, Snellings G, Cupers P, Craessaerts K, De Strooper B. Interaction with telencephalin and the amyloid precursor protein predicts a ring structure for presenilins. Neuron 20 November 2001;(32):579-589. Abstract

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  1. Another function for ps, also related to protein degradation but not like app or notch clevage.

    View all comments by Eddie Koo

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  1. . Interaction with telencephalin and the amyloid precursor protein predicts a ring structure for presenilins. Neuron. 2001 Nov 20;32(4):579-89. PubMed.

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  1. . Interaction with telencephalin and the amyloid precursor protein predicts a ring structure for presenilins. Neuron. 2001 Nov 20;32(4):579-89. PubMed.

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  1. . Interaction with telencephalin and the amyloid precursor protein predicts a ring structure for presenilins. Neuron. 2001 Nov 20;32(4):579-89. PubMed.