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amyloid staining

Printed From: Alzforum Bulletin Boards
Category: Desperately Seeking
Forum Name: Antibodies
Forum Discription: Desperately Seeking Antibodies
URL: http://www.alzforum.org/bboards/forum_posts.asp?TID=44
Printed Date: 24 May 2013 at 3:24pm


Topic: amyloid staining
Posted By: Paula Fernández
Subject: amyloid staining
Date Posted: 08 Oct 2007 at 5:48am
Good morning, all:
 
I would very much appreciate if you could inform me about amyloid stainings. I'm beggining a project on Alzheimer and I know that Congo Red and Thioflavin S staining are appropriate staining to see the amyloid plaques, but I don't know which one is more appropriate, neither the protocol to use these staining, neither how to see these stainings (fluorescence, light o polarized microscopy). I would need to know if these stainings work not using paraffin embebed tissue, but paraformaldhide fixed and freezed in OCT tissue. Do these staining work only when cut in 5 micrometer sections?
 
Thank you very much for your help.



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