14-3-3ζ
None
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human
immunogen = full-length human 14-3-3 zeta protein
28 kDa 14-3-3ζ
discontinued
14-3-3ζ/δ
None
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse
immunogen = NIH-recombinant (partial), N-terminal 14-3-3 protein
30 kDa 14-3-3ζ/δ
discontinued
14-3-3ε
LifeSpan Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, serum)
-
Bovine, Rabbit, Chicken, Sheep, Human, Mouse, Rat
immunogen = synthetic corresp. to C-terminal sequence of sheep 14-3-3ε
14-3-3ε (C-terminal, 30kDa)
-
14-3-3ε
LifeSpan Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, serum)
-
Bovine, Rabbit, Chicken, Sheep, Human, Mouse, Rat
immunogen = synthetic peptide corresp. to acetylated N-terminal sequence of sheep 14-3-3ε
14-3-3ε (N-terminal, 30kDa)
-
14-3-3ε
LifeSpan Biosciences, Inc.
polyclonal
IgG
Mouse
(liquid, PBS, unpurified serum)
-
Human
immunogen = human YWHAE (aa1-256) with GST Tag
14-3-3 protein epsilon (YWHAE)
-
14-3-3η
LifeSpan Biosciences, Inc.
polyclonal
IgG
Rabbit
(lyophilized, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to a region of human 14-3-3η (YWHAH)
14-3-3 protein eta(YWHAH)
-
14-3-3η
LifeSpan Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, serum)
-
Bovine, Rabbit, Chicken, Sheep, Human, Mouse, Rat
immunogen = synthetic peptide corresp. to acetylated N-terminal sequence of sheep 14-3-3 eta
14-3-3 protein eta(YWHAH)
-
14-3-3σ
LifeSpan Biosciences, Inc.
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, glycerol, no preservative, affinity purified)
-
Human
immunogen = recombinant human 14-3-3 sigma protein (26-30kD)
14-3-3 protein σ; no cross with other isoforms of 14-3-3
-
14-3-3σ
LifeSpan Biosciences, Inc.
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, glycerol, no preservative, affinity purified)
-
Human
immunogen = recombinant human 14-3-3 sigma protein (26-30kD)
14-3-3 protein σ; no cross with other isoforms of 14-3-3
-
14-3-3σ
LifeSpan Biosciences, Inc.
polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to aa1-248
14-3-3 protein σ; (Stratifin)
-
14-3-3σ
LifeSpan Biosciences, Inc.
monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide)
-
Human, Rat
immunogen = recombinant human 14-3-3 sigma protein (26-30kD)
14-3-3 protein σ
-
14-3-3σ
LifeSpan Biosciences, Inc.
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = GST fusion protein corresponding to full-length human 14-3-3 sigma
14-3-3 protein σ; no cross-reaction with other 14-3-3 isoforms
-
14-3-3σ
LifeSpan Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, serum)
-
Mammal (various), Bovine, Rabbit, Chicken, Sheep, Human, Mouse, Rat
immunogen = synthetic peptide corresp. to acetylated N-terminal sequence of sheep 14-3-3 sigma
14-3-3 protein σ (SFN)
-
14-3-3θ/τ
LifeSpan Biosciences, Inc.
monoclonal
IgG1
Mouse
(liquid, PBS, glycerol, azide, purified)
-
Bovine, Human, Mouse, Rat
immunogen = recombinant 14-3-3 theta/tau (a single protein which is known by the alternative names 14-3-3θ and 14-3-3τ)
14-3-3 proteinθ/τ; no cross-reaction with 14-3-3ζ
-
14-3-3τ
LifeSpan Biosciences, Inc.
monoclonal
IgG1
Mouse
-
Bovine, Human, Mouse, Rat
-
-
-
14-3-3τ
LifeSpan Biosciences, Inc.
polyclonal
IgG
Rabbit
(lyophilized, affinity purified)
-
Dog, Human, Mouse, Rat
immunogen = synthetic peptide corresp. to a region of human 14-3-3τ (YWHAQ)
14-3-3τ (YWHAQ)
-
14-3-3τ
LifeSpan Biosciences, Inc.
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, glycerol, no preservative, affinity purified)
-
Human
immunogen = recombinant protein of human 14-3-3 gamma (26-33kD)
recognizes beta, gamma, sigma and zeta isoforms of 14-3-3
-
14-3-3γ (HS23)
Novus Biologicals
monoclonal
IgG1 ,κ
Mouse
(liquid, ascites)
-
Human
immunogen = an N-terminal fragment of human 14-3-3 gamma
specific for human non-modified N-terminus 14-3-3 gamma; naturally occurring form of 14-3-3 gamma in which the N-terminus Met is removed and the Val is acetylated isnot recognized by this antibody
-
14-3-3γ (KC21)
Novus Biologicals
monoclonal
IgG2a
Mouse
(liquid, ascites)
-
Human
immunogen = an N-terminal fragment of human 14-3-3 gamma where the N-terminal Val was acetylated
specific for human 14-3-3 gamma; where the N-terminal Met is removed, resulting in an acetylated N-terminal Val. Unprocessed (non-modified) 14-3-3 gamma is not recognized by this antibody
-
14-3-3 binding motif, phos Ser (3i2)
US Biological
monoclonal
IgG1
Mouse
(liquid, ascites, HEPES, NaCl, BSA, glycerol, azide)
-
-
immunogen = synthetic phospho-(Ser) 14-3-3 binding motif peptides (KLH coupled)
wide range of peptides containing this phosphorylated 14-3-3 binding motif; no cross-reaction with corresponding non-phosphorylated sequences
-
14-3-3 binding motif, phos Ser
US Biological
polyclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
-
-
immunogen = synthetic phospho-(Ser) 14-3-3 binding motif peptides (KLH coupled)
binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position; no cross-reaction with corresp. non-phosphorylated sequences or with other phospho-Thr/Ser/Tyr -containing motifs
-
14-3-3ε (8j12)
US Biological
monoclonal
IgG2a , κ
Mouse
(liquid, PBS, azide, protein G affinity purified)
-
Human
immunogen = recombinant human 14-3-3 epsilon (1-255aa) purified from E. coli
wide range of peptides containing this phosphorylated 14-3-3 binding motif; no cross-reaction with corresponding non-phosphorylated sequences
-
14-3-3ε
LifeSpan Biosciences, Inc.
polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purfied)
-
Bovine, Human, Mouse, Rat
immunogen = recombinant protein from human YWHAE (aa1-255)
14-3-3 protein epsilon (YWHAE)
-
14-3-3σ
LifeSpan Biosciences, Inc.
polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purified)
-
Human, Mouse, Rat
immunogen = recombinant protein corresp. to aa1-248 from human SFN
14-3-3 protein σ; (Stratifin)
-
14-3-3τ
LifeSpan Biosciences, Inc.
monoclonal
IgG
Mouse
(liquid, PBS, BSA, glycerol, azide, affinity purified)
-
Dog, Rabbit, Human, Monkey, Mouse, Rat
-
recognizes a ~31 kDa protein, corresponding to the apparent molecular weight of the beta and zeta isoforms of 14-3-3
-
14-3-3γ (3F7)
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, no preservative, no BSA, protein G purified)
-
Human, Rat
immunogen = recombinant full length human 14-3-3 gamma protein
14-3-3 protein (beta, gamma and zeta isoforms)
-
14-3-3
Abcam
polyclonal
IgG
Rabbit
(liquid, whole antiserum, PBS, azide, BSA, thimerisol)
-
Human, Mouse, Rat
immunogen = a mixture of 14-3-3 fusion proteins (beta, eta, tau, and sigma)
14-3-3 isoforms
-
14-3-3
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide with the sequence D K S E L V Q K A K L A E Q A E R Y D, mapping to the N-terminal domain of human 14-3-3
14-3-3 isoforms (alpha, beta, zeta, and theta)
-
14-3-3 phos Ser (3i2)
Abcam
monoclonal
IgG1
Mouse
(liquid, HEPES, NaCl, glycerol, BSA, no preservative, protein A purified)
-
-
immunogen = synthetic phospho(Ser) 14-3-3 binding motif peptides (KLH coupled)
binds peptides/ proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresp. nonphosphorylated seq.
-
14-3-3
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Cow, Human, Mouse, Rat, Xenopus, C. elegans
immunogen = 20 aa synthetic peptide derived from the N-terminus of the human 14-3-3 beta/alpha protein
broadly reactive with members of the 14-3-3 family of proteins (~29–32kDa)
-
14-3-3 phos Ser58
Abcam
polyclonal
IgG
Rabbit
(liquid, HEPES, BSA, NaCl, no preservative, glycerol, affinity purified)
-
Human, Rat
immunogen = phosphopeptide corresp. to aa residues surrounding the phosphorylation site Ser58 of rat 14-3-3 protein
specific for the ~29k 14-3-3 protein phosphorylated at Ser58
-
14-3-3
Abcam
polyclonal
IgG
Rabbit
(liquid, HEPES, BSA, NaCl, no preservative, glycerol, affinity purified)
-
Human, Mouse, Rat, Xenopus
immunogen = synthetic peptide corresp. to aa residues from the C-terminal region of human 14-3-3 protein, conj. to KLH
14-3-3 protein
-
14-3-3
Abcam
polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purified)
-
Human, Mouse, Rat
immunogen = full length E coli -derived fusion protein
14-3-3 protein
-
14-3-3 (3F7)
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, BSA, azide, protein G purified)
-
Human
immunogen = recombinant full length human 14-3-3 gamma protein
14-3-3 protein
-
14-3-3
Abcam
polyclonal
IgG
Sheep
(liquid, PBS, azide, protein G purified)
-
Human
immunogen = full length native 14-3-3 protein purified from human brain
recognises the lower molecular weight subunit (26 kDa) of 14-3-3
-
14-3-3 pan (2Q248)
Abcam
monoclonal
IgG2a
Mouse
(liquid, supernatant, no preservative)
-
Rat
immunogen = synthetic peptide, corresp. to aa208-244 of rat 14-3-3
14-3-3 protein (33 kDa)
-
14-3-3α/β (Y62)
Abcam
monoclonal
IgG
Rabbit
(liquid, tris-glycine, NaCl, BSA, glycerol, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to residues in the C-terminal of human 14-3-3 alpha + beta
specific for 14-3-3 alpha + beta
-
14-3-3η
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, no preservative, sucrose, affinity purified)
-
Human
immunogen = synthetic peptide, corresp. to a region between aa71-120 of human 14-3-3η
14-3-3eta (28kDa)
-
14-3-3γ
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide derived from internal of human 14-3-3 gamma
detects endogenous levels of total 14-3-3 gamma protein
-
14-3-3ζ/δ
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, NaCl, azide, affinity purified)
-
Human, Mouse
immunogen = synthetic non-phosphopeptide derived from human 14-3-3 zeta + delta around the phosphorylation site of threonine 232 (S-D-TP-Q-G)
detects endogenous levels of total 14-3-3 zeta + delta protein.
-
14-3-3α/β
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, protein G purified)
-
Human
immunogen = synthetic peptide selected within aa 200~300 of human 14-3-3 beta, conj. KLH
binds both transcript variants of 14-3-3 (alpha and beta)
-
14-3-3α/β
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, protein G purified)
-
Human
immunogen = synthetic peptide selected within aa 50~150 of human 14-3-3 beta (N-terminal), conj. KLH
binds to both isoforms of 14-3-3 (alpha and beta)
-
14-3-3α/β (E58)
Abcam
monoclonal
IgG
Rabbit
(liquid, Ig fract., tris-glycine, NaCl, BSA, glycerol, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to residues in the N-terminal of human 14-3-3 alpha + beta
specific for 14-3-3 alpha + beta
-
14-3-3α/β (22-IID8B)
Abcam
monoclonal
IgG
Rabbit
(liquid, PBS, PMSF, glycerol, no preservative, protein G purified)
-
Dog, Rabbit, Cow, Sheep, Pig, Human, Monkey, Mouse, Rat
immunogen = lysed transformed amnion cells (AMA) (human)
specific for 14-3-3 alpha + beta
-
14-3-3 Binding Domain (phos S)
Abcam
polyclonal
IgG
Rabbit
(liquid, HEPES, BSA, NaCl, no preservative, glycerol, protein A purified)
-
-
immunogen = synthetic phospho (Ser) 14-3-3-binding-motif peptides
binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position
-
14-3-3ε
Abcam
polyclonal
IgG
Rabbit
(liquid, whole antiserum)
-
Rabbit, Chicken, Cow, Sheep, Human, Mouse, Rat
immunogen = synthetic peptide from within N-terminus of 14-3-3 epsilon
14-3-3epsilon; may not react with recombinant protein expressed in E. coli that is not N-acetylated
-
14-3-3η
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide (human) from an internal region
detects endogenous levels of total 14-3-3eta
-
14-3-3γ (3F8)
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, no preservative, protein G purified)
-
Human
immunogen = recombinant full length human 14-3-3 gamma protein
14-3-3γ; no crossreactivity with other isoforms of 14-3-3
-
14-3-3ζ phos Ser58
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, NaCl, glycerol, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic phosphopeptide from human 14-3-3-zeta, around the phosphorylation site of serine 58 (R-S-pS-W-R)
endogenous levels of 14-3-3 zeta only when phosphorylated at serine 58 (28kDa)
-
14-3-3ζ/δ
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, protein G purified)
-
Human
immunogen = synthetic peptide selected within aa 200-300 of human 14-3-3 zeta (C-terminal)
specific for 14-3-3 zeta + delta
-
14-3-3ζ
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized non-phosphopeptide derived from human 14-3-3 zeta around the phosphorylation site of serine 58 (R-S-Sp-W-R)
endogenous levels of total 14-3-3 zeta protein
-
14-3-3β
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, BSA, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide (unfortunately, the aa seq. is considered to be commercially sensitive) (human) (N-terminal)
14-3-3beta
-
14-3-3β (60C10)
Abcam
monoclonal
IgG2b , κ
Mouse
(liquid, PBS, no preservative, glycerol, protein A purified)
-
Human, Mouse, Rat
immunogen = recombinant full length human protein
14-3-3beta
-
14-3-3ε
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, NaCl, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide from C-terminus of human 14-3-3 epsilon
detects endogenous levels of total 14-3-3 epsilon protein
-
14-3-3ε
Abcam
polyclonal
IgG
Rabbit
(liquid, whole antiserum)
-
Rabbit, Chicken, Cow, Sheep, Human, Mouse, Rat
immunogen = synthetic peptide from C-terminus of 14-3-3 epsilon
14-3-3epsilon
-
14-3-3ε
Abcam
polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purified)
-
Cow, Human, Mouse, Rat
immunogen = full length protein human 14-3-3 epsilon
14-3-3epsilon
-
14-3-3ε (5A5)
Abcam
monoclonal
IgG2a , κ
Mouse
(liquid, PBS, azide, protein G purified)
-
Human
immunogen = recombinant full length human protein purified from E. coli
14-3-3epsilon
-
14-3-3σ (1433S01)
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, no BSA, glycerol, no preservative protein G purified)
-
Human
immunogen = recombinant full length human 14-3-3σ
specific to 14-3-3 sigma; shows no cross-reaction with other isoforms of 14-3-3
-
14-3-3σ
Abcam
polyclonal
IgG
Rabbit
(liquid, whole serum, azide)
-
Human
immunogen = synthetic peptide: S N E E G S E E K G P E V, corresp. to internal seq. aa69-81 of human 14-3-3 sigma
specific to 14-3-3 sigma
-
14-3-3σ
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified)
-
Human, Mouse
immunogen = synthetic peptide human 14-3-3 sigma
detects endogenous levels of total 14-3-3 sigma protein
-
14-3-3σ
Abcam
polyclonal
IgG
Goat
(liquid, TBS, BSA, azide, affinity purified)
-
Human
immunogen = synthetic peptide: D L H T L S E D S Y K D S T from the internal region of human 14-3-3 sigma protein seq. (NP_006133.1)
14-3-3 sigma protein
-
14-3-3σ (1.N.6)
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, BSA, glycerol, azide, protein G purified)
-
Human, Mouse
immunogen = recombinant full length protein (human)
recognizes human 14-3-3 sigma protein, 26-30kD; no crossreaction with other isoforms of 14-3-3
-
14-3-3σ (1.T.28)
Abcam
monoclonal
IgG1
Mouse
(liquid, Tris-glycine, NaCl, glycerol, azide, protein G purified)
-
Human, Mouse, Rat
immunogen = recombinant full length human 14-3-3σ
recognizes 14-3-3 sigma and does not crossreact with other 14-3-3 isoforms. It has been specifically tested against 14-3-3 gamma, beta, zeta, and epsilon
-
14-3-3σ (SPM159)
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, BSA, azide, protein G purified, prediluted)
-
Human
immunogen = recombinant human 14-3-3σ
specific to 14-3-3 sigma
-
14-3-3τ (3B9)
Abcam
monoclonal
IgG1
Mouse
(liquid, PBS, glycerol, azide, protein G purified)
-
Cow, Human, Mouse, Rat
immunogen = recombinant full length protein (human)
recognises 14-3-3-theta/tau isoform; no reaction with the 14-3-3 zeta isoform
-
14-3-3τ phos Ser232
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, NaCl, affinity purified)
-
Human, Mouse
immunogen = synthetic phosphopeptide derived from human 14-3-3 Tau around the phosphorylation site of serine 232 (S-D-Sp-A-G)
detects 14-3-3 Tau only when phosphorylated at serine 232
-
14-3-3τ
Abcam
polyclonal
IgG
Mouse
(liquid, whole serum, glycerol, no preservative)
-
Mouse
immunogen = vector coding for a partial recombinant fusion protein, corresp. to aa51-151 of mouse 14-3-3 Tau
detects 14-3-3 Tau
-
14-3-3β
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, no preservative, glycerol, protein A purified)
-
Human, Mouse, Rat
immunogen = recombinant full length protein (human)
14-3-3beta
-
14-3-3γ (CG31)
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, no preservative, no BSA, protein G purified)
-
Human
immunogen = recombinant full length human 14-3-3 gamma protein
14-3-3γ; no crossreactivity with other isoforms of 14-3-3
-
14-3-3τ
Abcam
polyclonal
IgG
Rabbit
(liquid, whole antiserum, azide)
-
Chicken, Cow, Sheep, Human, Mouse, Rat
immunogen = synthetic peptide corresp. to acetylated N- terminal seq. of sheep 14-3-3 Tau
recognises 14-3-3 tau; may not react with recombinant proteins that are not N-acetylated
-
14-3-3τ
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, NaCl, azide, affinity purified)
-
Human, Mouse
immunogen = synthetic non-phosphopeptide derived from human 14-3-3 Tau near phosphorylation site of serine 232 (S-D-Sp-A-G)
recognises 14-3-3 tau; may not react with recombinant proteins that are not N-acetylated
-
14-3-3τ
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, NaCl, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide derived from internal sequence of human 14-3-3 Tau
detects endogenous levels of total 14-3-3 Tau protein
-
14-3-3τ
Abcam
polyclonal
IgG
Goat
(liquid, TBS, BSA, azide, affinity purified)
-
Human
immunogen = Synthetic peptide: C-D D R K Q T I D N S Q, corresp. to internal seq. aa136-146 of human 14-3-3 Tau (NP_006817.1)
detects endogenous levels of total 14-3-3 Tau protein
-
14-3-3ζ/δ phos T232
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified)
-
Human, Mouse
immunogen = synthetic phosphopeptide derived from human 14-3-3 zeta + delta around the phosphorylation site of threonine 232 (S-D-Tp-Q-G)
detects endogenous levels of 14-3-3 zeta + delta only when phosphorylated at threonine 232
-
14-3-3ζ
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide: A G D D K K G I V D Q S Q Q A Y, corresp. to aa134 - 149 of human 14-3-3 zeta
specific for 14-3-3 zeta (30kDa)
-
14-3-3ζ
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide from an internal region of human 14-3-3 zeta
specific for 14-3-3 zeta (30kDa)
-
14-3-3α/β
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Cow, Human, Mouse, Rat, Xenopus, C. elegans
immunogen = synthetic peptide derived from the N-terminus of human 14-3-3 alpha/beta protein
broadly reactive with the ~29-32 kDa members of the 14-3-3 family of proteins
-
14-3-3β/ζ (4E2)
Abcam
monoclonal
IgG
Mouse
(liquid, PBS, no preservative, glycerol, PMSF, protein G purified)
-
Dog, Cow, Sheep, Pig, Human, Monkey, Mouse, Rat
immunogen = tissue / cell preparation (lysed transformed human amnion cells)
14-3-3beta/zeta
-
14-3-3β (4E1)
Abcam
monoclonal
IgG2b , κ
Mouse
(liquid, PBS, azide, sucrose, protein A purified)
-
Mouse, Rat
immunogen = recombinant full length rat protein
14-3-3beta
-
14-3-3β
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, BSA, affinity purified, prediluted)
-
Human, Mouse, Rat
immunogen = synthetic peptide (unfortunately, the aa seq. is considered to be commercially sensitive) (human) (N-terminal)
14-3-3beta
-
14-3-3η
Abcam
polyclonal
IgG2a , κ
Rabbit
(liquid, PBS, no preservative, protein G purified)
-
Human
immunogen = recombinant fragment corresp. to aa71-170 of human 14-3-3 eta
14-3-3eta
-
14-3-3γ
Abcam
monoclonal
IgG2b , κ
Mouse
(liquid, PBS, no preservative, protein G purified)
-
Human
immunogen = recombinant fragment corresp. to aa70-170 of human 14-3-3 gamma
14-3-3γ
-
14-3-3γ
Abcam
polyclonal
IgG
Rabbit
(liquid, whole antiserum, no preservative)
-
Chicken, Cow, Sheep, Human, Mouse, Rat
immunogen = synthetic peptide from the N-terminal area of sheep 14-3-3 gamma
14-3-3 gamma protein
-
14-3-3γ (3F8)
Abcam
monoclonal
IgG, κ
Mouse
(liquid, PBS, BSA, azide, protein G purified)
-
Human
immunogen = recombinant full length human 14-3-3 gamma protein
recognizes both zeta and gamma isoforms. This antibody shows no crossreactivity with other isoforms of 14-3-3
-
14-3-3τ/ϑ (3B9)
Abcam
monoclonal
IgG1
Mouse
(liquid, glycerol, azide, PBS, conj. agarose)
-
Cow, Human, Mouse, Rat
immunogen = recombinant full length protein (human)
reacts specifically with the Tau + Theta isoform and does not react with 14-3-3 zeta
-
14-3-3τ/ϑ (3B9)
Abcam
monoclonal
IgG1
Mouse
(liquid, glycerol, azide, PBS, conj. HRP)
-
Cow, Human, Mouse, Rat
immunogen = recombinant full length protein (human)
reacts specifically with the Tau + Theta isoform and does not react with 14-3-3 zeta
-
14-3-3ζ/δ
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse
immunogen = recombinant frag., corresp. to N-terminal aa1-150 of 14-3-3 zeta
specific for 14-3-3 zeta + delta
-
14-3-3ζ
Abcam
polyclonal
IgG
Mouse
(liquid, whole serum, glycerol, no preservative)
-
Mouse
immunogen = recombinant fusion protein corresp. to aa51-150 of mouse 14-3-3 zeta
specific for 14-3-3 zeta (30kDa)
-
14-3-3ζ (8C3)
Abcam
monoclonal
IgG2a
Mouse
(liquid, ascites)
-
Sheep, Rat
immunogen = tissue / cell preparation (sheep pineal gland)
14-3-3 zeta
-
14-3-3ζ
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified, prediluted)
-
Human, Mouse, Rat
immunogen = synthetic peptide: A G D D K K G I V D Q S Q Q A Y, corresp. to aa134 - 149 of human 14-3-3 zeta
specific for 14-3-3 zeta
-
14-3-3β/ζ phos ser185
Abcam
polyclonal
IgG
Sheep
(liquid, BSA, azide, glycerol, TBS, affinity purified)
-
Cow, Sheep, Human, Mouse, Rat
immunogen = synthetic phosphopeptide corresp. to aa surrounding phospho Serine185
recognizes 14-3-3 phospho Serine185. It is specific for the phosphorylated form of beta and zeta 14-3-3 that are found endogenously phosphorylated only in brain tissue
-
14-3-3η/γ/ζ
Abcam
polyclonal
IgG
Rabbit
(liquid, whole serum, no preservative)
-
Mammal (various)
immunogen = Synthetic peptide: K M K G D Y Y R Y L A E, corresp. to aa124-135 of 14-3-3 eta + gamma + zeta
recognizes eta, gamma and zeta isoforms at 27kDa
-
14-3-3η/γ/ζ
Abcam
polyclonal
IgG
Rabbit
(liquid, whole serum, no preservative)
-
Various vertebrate
immunogen = HPLC purified synthetic peptide (K M K G D Y Y R Y L A E) corresp. to the zeta, gamma and eta isoforms of 14-3-3
recognizes eta, gamma and zeta isoforms
-
14-3-3η
Abcam
polyclonal
IgG
Rabbit
(liquid, whole serum, no preservative)
-
Rabbit, Chicken, Cow, Sheep, Human, Mouse, Rat
immunogen = synthetic peptide from the N-terminal area of sheep 14-3-3 eta
14-3-3eta
-
14-3-3 (aC-12)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Arabidopsis thaliana
immunogen = epitope mapping near the C-terminus of Arabidopsis thaliana 14-3-3
14-3-3
-
14-3-3 (at-82)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Arabidopsis thaliana
immunogen = epitope corresp. to aa 21-75 mapping within an internal region of Arabidopsis thaliana 14-3-3
14-3-3 and to a lesser extent 14-3-3-like proteins
-
14-3-3 (B-11), pan
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = raised against aa 1-246 representing full length human 14-3-3β
14-3-3
-
14-3-3 (B-8), pan
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = raised against aa 1-246 representing full length human 14-3-3β
14-3-3
-
14-3-3 (Fl-246), pan
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = raised against aa 1-246 representing full length human 14-3-3β
14-3-3 protein family
-
14-3-3 (K-19), pan
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope mapping at the N-terminus of human 14-3-3β
detects members of 14-3-3 protein family
-