digoxigenin, Fab fragments
Roche Applied Science
polyclonal
Sheep
(IgG waspurified and and specificIgG was isolated by immunosorption. Fab fragments wereobtained by papain digestion, purified, PBS, BSA, lyophilized)
-
-
immunogen = digoxigenin
used for the detection of digoxigenin- labeled compounds.
-
FLAG (M2)
Sigma Aldrich
monoclonal
IgG1
Mouse
(purified Ig, buffered aqueous glycerol solution, PBS, BSA, preservative, Cy3™)
-
Various species, Mouse, Rat
-
Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent; useful in detection of FLAG fusion proteins expressed in murine host
-
FLAG (M2)
Sigma Aldrich
monoclonal
IgG1
Mouse
(High Sensitivity, M2 coated 96-well plates)
-
Various species
-
Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Suitable for screening for expression, study of protein:protein interactions and ELISA assays
-
myc
Invitrogen Corporation
monoclonal
IgG1
Mouse
(liquid, PBS, azide)
-
-
immunogen = the c-myc epitope (recognizes the sequence:-Glu -Gln -Lys -Leu -Ile -Ser -Glu -Glu -Asp -Leu- )
for detection of recomb. proteins containing the c-myc epitope
-
c-Myc
Sigma Aldrich
polyclonal
Rabbit
(agarose affinity gel, PBS, azide)
-
-
immunogen = synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein (Glu-Gln-Lys-Leu-Ile- Ser-Glu-Glu-Asp-Leu)
N-terminal or C-terminal c-Myc tagged fusion proteins
-
c-Myc
Sigma Aldrich
polyclonal
Rabbit
(buffered aqueous solution, PBS, BSA, azide)
-
-
-
N-terminal or C-terminal c-Myc tagged fusion proteins
-
c-Myc
Sigma Aldrich
polyclonal
Rabbit
(prepared by the two-step glutaraldehyde method; buffered aqueous solution, PBS, thimerosal, Peroxidase)
-
-
-
N-terminal or C-terminal c-Myc tagged fusion proteins
-
HA-Agarose (HA-7)
Sigma Aldrich
monoclonal
IgG1
Mouse
(liquid, purified, PBS suspension, azide, agarose; also available as purified Ig, H3663; glycerol, A5477;ascites, H9658;biotin, B9183;FITC, H7411;Peroxidase, H6533;TRITC, H9037)
-
-
immunogen = synthetic peptide corresp. to aa residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA), conjugated to KLH
Reacts with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells
-
polyHIS-Agarose (HIS-1)
Sigma Aldrich
monoclonal
IgG2a
Mouse
(liquid, purified, PBS suspension, azide, agarose;)
-
-
immunogen = recombinant polyHistidine tagged fusion protein
recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors
-
ECS
Bethyl Labs
polyclonal
Rabbit
(affinity isolated, PBS, azide;also available as affinity purified and HRP-conj., A190-102P)
-
-
immunogen = ECS, Enterokinase Cleavage Site (xxxDDDDK) conjugated to KLH
reacts with FLAGTM
-
ECS
Bethyl Labs
polyclonal
Rabbit
(phosphate, saline, azide)
-
-
immunogen = ECS, Enterokinase Cleavage Site (xxxDDDDK) conjugated to KLH
reacts with FLAGTM
-
Biotin
Bethyl Labs
polyclonal
Rabbit
(liquid, affinity purified, phosphate, saline, azide;also available HRP conj., A150-109P)
-
-
-
reacts specifically with KLH-biotin and BSAbiotin.There is no reaction with free KLH or BSA.
-
Penta-HIS
QIAGEN Inc.
monoclonal
IgG
Mouse
(liquid, stabilized stock solution, Biotin conj.)
-
-
-
detects five consecutive histidine residues. Negligible cross with crude E. coli, yeast, mammalian, or insect cell lysates
-
IgG
Calbiochem EMD Biosciences
unknown
Unknown Host
(liquid slurry, PBS, azide, agarose conj., suspension, ready to use)
-
-
-
covalently coupled to the agarose support and does not leach. The protein A will bind IgG from biological fluids, but with different efficiencies, depending on the species. In general, Protein A works well for purification of IgG of large mammals, while Protein G PLUS and a mixture of Protein G PLUS with Protein A are best for rodent IgG purifications
-
IgG
Calbiochem EMD Biosciences
unknown
Unknown Host
(liquid slurry, PBS, azide, agarose suspension, ready to use)
-
-
-
designed for IP applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification
-
IgG
Calbiochem EMD Biosciences
unknown
Unknown Host
(liquid slurry, PBS, azide, agarose conj., suspension, ready to use)
-
-
-
designed for purification of IgG from biological fluids The protein is covalently coupled to the agarose support and does not leach. The protein A will bind IgG from biological fluids, but with different efficiencies, depending on the species. In general, Protein A works well for purification of IgG of large mammals, while Protein G PLUS and a mixture of Protein G PLUS with Protein A are best for rodent IgG purifications
-
IgG
Calbiochem EMD Biosciences
unknown
Unknown Host
(liquid slurry, PBS, azide, agarose conj., suspension, ready to use)
-
-
-
has multiple Ig binding sites genetically engineered for higher binding capacity of mouse IgG1 or IgG3 as well as IgG from rabbit, rat, and other species. Mouse IgG2a or IgG2b bind moderately to Protein G PLUS
-
GST
Invitrogen Corporation
polyclonal
IgG
Rabbit
(antiserum, affinity purified, PBS, azide,)
-
-
Immunogen = recombi.t GST protein from Schistosoma japonicum PAD KG2
Specific for the ~26 kDa GST protein. Useful for detecting GST and GST-fusion proteins transcribed from pGEX and similar vectors
-
digoxigenin (1.71.256)
Roche Applied Science
monoclonal
IgG1 ,κ
Mouse
(purified by gel filtration, phosphate buffer, saline, MIT, lyophilized)
-
-
immunogen = digoxigenin
reacts with free and bound digoxigenin
-
FLAG (2EL-1B11)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, ascites, no preservative)
-
Various vertebrate
immunogen = Synthetic peptide (Cys)-DYKDDDK, coupled to ovalbumin
Reacts with both N-terminal and C-terminal fusion proteins and does not require Ca++ for binding. Reacts better in most cases than M2 or M4 FLAG clones
-
GST (Z-5)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatinalso available as:sc-459 FITC,sc-459 B, sc-459 AF405,sc-459 AF459,sc-459 AF647)
-
Human
Immunogen = a sequence of GSTof Schistosoma japonicum origin
recognizes 26 kDa glutathione S-transferase (GST) of Schistosoma japonicum origin and GST fusion proteins
-
GFP
MBL International
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, BSA, azide, purified)
-
Non-specific
-
reacts with wild-type GFP, and its variants EGFP and EBFP, etc.
-
polyHistidine (HIS-1)
Sigma Aldrich
monoclonal
IgG2a
Mouse
(liquid, ascites;also available asPeridoxase, A7058;Alkaline Phosphatase, A5588)
-
-
immunogen = recombinant polyHistidine tagged fusion protein
recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors
-
c-Myc (9E10)
Santa Cruz
monoclonal
IgG1
Mouse
(gelatin, PBS, azide, agarose, conj)
-
Human, Mouse, Rat
immunogen = peptide corresp. to aa 408-439 within the C-terminal domain of c-Myc of human origin
detects c-Myc p67
-
HA tag
Upstate and Chemicon are Millipore
monoclonal
IgG3
Mouse
(liquid, protein A purified, PBS, azide)
-
-
immunogen = KLH-conjugated, synthetic peptidecorresponding to amino acids 98-106 (YPYDVPDYA)of influenza hemaglutinin
Recognizes HA tagged proteinsoverexpressed in cells, including both amino orcarboxy-termini of targeted proteins in transfectedmammalian cells
-
Cre Recombinase (2D8)
Chemicon and Upstate are Millipore
monoclonal
IgG1 ,κ
Mouse
(liquid, ascites, azide, unpurified, no preservatives)
-
Non-specific
immunogen = Cre recombinase (aa 77 to 343) fusion protein
recognizes Cre recombinase, ~38 kDa
-
Cre Recombinase (7.23)
BioLegend
monoclonal
IgG1
Mouse
(purified)
-
Non-specific
immunogen = affinity purified cre recombinase
reacts with Cre Recombinase; is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It can be used to determine the success of gene manipulations in transgenic animals
Available from BioLegend
Cre Recombinase
None
polyclonal
Rabbit
(liquid, antiserum)
-
Non-specific
immunogen = purified full-length cre recombinase
reacts with Cre Recombinase; highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It can be used to determine the success of gene manipulations in transgenic animals
Discontinued
c-Myc (9E10)
ATCC
monoclonal
IgG1
Mouse
(frozen suspension)
-
Human
immunogen = synthetic peptide derived using the sequence of the human c-myc gene product
detects c-Myc
-
HA (3F10)
Roche Applied Science
monoclonal
IgG1
Rat
(purified from bioreactor supernatants and lyophilized.Also available asBiotin, 12158167001Peroxidase, 12013819001)
-
-
immunogen = synthetic peptide (residues 76-111 of X47 hemaglutinin 1) coupled to keywhole limpet hemocyanin(KLH)
for the detection of native and recombinant “epitope tagged” HA proteins; recognizes the HA peptide sequence (YPYDVPDYA) derived from the influenza hemagglutinin protein
-
c-myc (9E10)
BioLegend
monoclonal
IgG1 ,κ
Mouse
(affinity purified, PBS, azide)
-
Non-specific
immunogen = amino acids 408-439, C-terminal region of human c-myc
reacts with human and fusion proteins in all species
-
myc Tag (9E10)
Upstate and Chemicon are Millipore
monoclonal
IgG1
Mouse
(liquid, glycerol, Tris-glycine, saline, azide, protein G purified)
-
Non-specific
immunogen = synthetic peptide corresponding to amino acids 408-437 of the Leucine zipper region of human Myc
recognizes Myc and recombinant proteins containing the Myc epitope tag
-
V5
Invitrogen Corporation
monoclonal
IgG2a
Mouse
(liquid, purified, PBS, HRP conj.)
-
-
immunogen = the V5 epitope (recognizes the sequence:Gly-Lys-Pro-Ile- Pro- Asn-Pro-Leu-Leu-Gly- Leu-Asp-Ser-Thr- )
detects recombinant proteins containing the 14 amino acid V5 epitope.
-
V5
Invitrogen Corporation
monoclonal
IgG2a
Mouse
(liquid, purified, PBS, azide)
-
-
immunogen = the V5 epitope (recognizes the sequence:Gly-Lys-Pro-Ile- Pro- Asn-Pro-Leu-Leu-Gly- Leu-Asp-Ser-Thr- )
detects recombinant proteins containing the 14 amino acid V5 epitope.
-
GFP
Abcam
polyclonal
IgG
Rabbit
(liquid, Tris HCl, glycerol, no preservative, affinity purified)
-
Non-specific
-
reacts with GFP
-
c-Myc (JAC6)
AbD Serotec
monoclonal
IgG1
Rat
(liquid, affinity purified IgG, PBS, azide)
-
Human
immunogen = residues 408-439 corresp. to the C-terminal region of human c-myc
recognises the myc epitope which is frequently used to tag proteins, and is the same epitope recognised by clone 9E10
-
digoxigenin
Roche Applied Science
polyclonal
Sheep
(lyophilized. sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. phosphate buffer, saline, MIT)
-
-
immunogen = digoxigenin
100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens or androgens
-
digoxigenin-AP, Fab fragments
Roche Applied Science
polyclonal
Sheep
(stabilized solution. IgG waspurified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer)
-
-
immunogen = digoxigenin
100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens or androgens
-
HA-probe (153)
Santa Cruz
monoclonal
IgG2a
Rat
(liquid, PBS, azide, gelatinAlso available as phycoerythrin (sc-53516 PE) or fluorescein (sc-53516 FITC)conjugates for flow cytometry)
-
Various species
Immunogen = HA peptide derived from influenza hemaglutinin
for detection of proteins containing theHA tag
-
HA-probe (F-7)
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin.also available as: sc-7392 AC;sc-7392 B;sc-7392 PE;sc-7392 HRP;sc-7392 FITC;sc-7392 TRITC;Alexa Fluor®s and TransCruz reagent for ChIP, sc-7392 X)
-
Various species
Immunogen = an internal region of the influenza hemaglutinin (HA) protein
for detection of proteins containing theHA tag
-
HA-probe (HA.C5)
Santa Cruz
monoclonal
IgG3
Mouse
(liquid, PBS, azide, gelatin)
-
Various species
Immunogen = a synthetic peptide derived from the human influenza hemaglutinin
for detection of proteins containing theHA tag
-
HA-probe (A00087.01)
Santa Cruz
monoclonal
IgG3
Mouse
(liquid, PBS, azide, gelatin)
-
Various species
Immunogen = synthetic peptide derived from influenza hemaglutinin
for detection of proteins containing theHA tag
-
DYKDDDDK tag
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, antisera, phosphate, saline, azide, affinity purifued)
-
Human, Mouse, Rat
immunogen = keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK
specific for the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp- Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins
-
GFP
Novus Biologicals
polyclonal
IgG
Rabbit
(whole antisera, phosphate, saline, azide)
-
-
immunogen = highly purified recombinant full length protein made in E. coli. The antibody is directed against the entire GFP molecule
reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP
-
GFP
Novus Biologicals
polyclonal
IgG
Rabbit
(whole antisera, phosphate, saline, azide)
-
-
immunogen = GFP fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria
reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP
-
GST
Novus Biologicals
polyclonal
IgG
Goat
(liquid, antisera, affinity purified, PBS, azide,)
-
-
Immunogen = GST protein from Schistosoma japonicum
recognizes Glutathione-S-Transferase (GST)
-
Histidine Tag (AD1.1.10)
Novus Biologicals
monoclonal
IgG1
Mouse
(protein-G purified ascites, PBS, azide, Biotin conj.)
-
-
immunogen = PAX6 transcription factor linked to histidine tag
recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags; against all His tagged proteins so far tested
-
Goat IgG (H and L)
Novus Biologicals
polyclonal
Rabbit
(liquid, antisera, PBS, Pro-Clean 400, HRP conj, affinity purified)
-
-
Immunogen = Goat IgG whole molecule
-
-
V5
Novus Biologicals
polyclonal
Rabbit
(affinity purified, PBS, azide)
-
Non-specific
immunogen = synthetic peptide representing amino acid residues 95 to 108 of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH
recognizes the V5 cleavage site
-
c-myc
Novus Biologicals
polyclonal
Rabbit
(affinity purified, PBS, azide)
-
Non-specific
immunogen = c-myc (EQKLISEEDL) conjugated to KLH
recognizes c-myc
-
HA (HA-7)
Sigma Aldrich
monoclonal
IgG1
Mouse
(liquid, purified IgG, PBS, BSA, azide)
-
-
immunogen = synthetic peptide corresp. to aa residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA), conjugated to KLH
Reacts with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells
-
HA (HA-7)
Sigma Aldrich
monoclonal
IgG1
Mouse
(ascites fluid, azide)
-
-
immunogen = synthetic peptide corresp. to aa residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA), conjugated to KLH
Reacts with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells
-
Digoxigenin (21H8)
Novus Biologicals
monoclonal
IgG1 , κ
Mouse
(liquid, ascites, glycerol, BSA, PBS, Thimerosal, purified, conj. biotin)
-
Non-specific
-
reacts with free and bound digoxigenin
-
Digoxigenin (21H8)
Novus Biologicals
monoclonal
IgG1 , κ
Mouse
(liquid, ascites, no preservative)
-
Non-specific
-
reacts with free and bound digoxigenin
-
Digoxigenin (21H8)
Novus Biologicals
monoclonal
IgG1 , κ
Mouse
(liquid, purified ascites, PBS, BSA, glycerol, thimerosal, conj. HRP)
-
Non-specific
-
reacts with free and bound digoxigenin
-
Digoxigenin
Novus Biologicals
polyclonal
IgG
Sheep
(liquid, antiserum, PBS, azide, protein G purified)
-
-
immunogen = digoxin-3-CMO conjugated to KLH
digoxigenin; cross reacts with digoxin and digitoxin; negligible reactivity has been observed with Ouabain
-
Digoxigenin (21H8)
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, ascites, BSA, glycerol, PBS, no preservative)
-
Non-specific
-
reacts with free and bound digoxigenin
-
Digoxigenin (21H8)
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, BSA, glycerol, PBS, thimerosal, conj. biotin)
-
Non-specific
-
reacts with free and bound digoxigenin
-
Digoxigenin (21H8)
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, purified ascites, PBS, BSA, glycerol, thimerosal, conj. HRP)
-
Non-specific
-
reacts with free and bound digoxigenin
-
Digoxigenin
Abcam
polyclonal
IgG
Sheep
(liquid, antiserum, PBS, azide, protein G purified)
-
-
immunogen = digoxin-3-CMO conjugated to KLH
Digoxigenin
-
Digoxigenin
Abcam
polyclonal
IgG
Goat
(liquid, PBS, azide, ion exchange chromatography)
-
-
immunogen = digoxigenin conj. to KLH
Digoxigenin and digoxin; no cross-reaction with the carrier protein KLH or other steroids
-
Digoxigenin
Abcam
polyclonal
IgY
Chicken
(liquid, BSA, thimerosal, Hepes-saline, affinity purified, conj. HRP)
-
-
immunogen = digoxigenin conj. to KLH
Digoxigenin
-
Digoxigenin
Abcam
polyclonal
IgY
Chicken
(liquid, PBS, azide, affinity purified)
-
-
immunogen = digoxigenin conj. to KLH
Digoxigenin
-
Digoxigenin (21H8)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, ascites, azide)
-
Non-specific
immunogen = Digoxigenin
reacts with free and bound digoxigenin
-
Digoxigenin (1.A2.1)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, ascites, azide)
-
Non-specific
immunogen = Digoxigenin
reacts with free and bound digoxigenin
-
Digoxigenin
LifeSpan Biosciences, Inc.
polyclonal
IgG
Sheep
(liquid, BSA, PBS, azide, affinity purified, modified Biomagnetic particle (BMP))
-
-
immunogen = Digoxigenin
Digoxigenin
-
Biotin (15D9)
Santa Cruz
monoclonal
IgG2b
Mouse
(liquid, affinity purified,)
-
-
immunogen = full length biotin
permits the formation of antibody-Biotin complexes, thus enhancing the sensitivity of the detection system; non cross-reactive with the free carrier protein
-
Biotin (212-01)
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, affinity purified,)
-
-
immunogen = biotinylated denatured Ovalbumin
permits the formation of antibody-Biotin complexes, thus enhancing the sensitivity of the detection system
-
Biotin (33)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, affinity purified,)
-
-
immunogen = biotin
detection of both free and carrier protein bound biotin; also recommended for detection of biocytin
-
Biotin (39-15D9)
Santa Cruz
monoclonal
IgG2b
Mouse
(liquid, ascites, azide, affinity purified)
-
-
immunogen = full length biotin
permits the formation of antibody-Biotin complexes, thus enhancing the sensitivity of the detection system; non cross-reactive with the free carrier protein
-
Biotin (3E6)
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, gelatin, azide)
-
-
immunogen = biotinylated denatured Ovalbumin
permits the formation of antibody-Biotin complexes, thus enhancing the sensitivity of the detection system
-
Biotin (BK-1/39)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, affinity purified)
-
-
immunogen = biotin conj. KLH
detection of biotin; permits the formation of antibody-Biotin complexes, thus enhancing the sensitivity of the detection system
-
Biotin (BIO-8)
Santa Cruz
monoclonal
IgG3
Mouse
(liquid, ascites, azide, BSA)
-
-
immunogen = biotin labeled DNA and biotin-labeled alkaline phosphatase
detection of biotin; permits the formation of antibody-Biotin complexes, thus enhancing the sensitivity of the detection system; non cross-reactive with Biotin, Digoxigenin, or BSA
-
GFP (FL)
Santa Cruz
polyclonal
IgG
Rabbit
(IgG, PBS, gelatin, azide;agarose conjugate for IP, sc-8334 AC,HRP conjugate for WB, sc-8334 HRP,biotin conjugate, sc-8334 B)
-
Non-specific
immunogen = peptide of amino acids 1-238representing full length GFP of Aequorea victoria origin
detection of GFP
-
HA.11
BioLegend
polyclonal
Rabbit
(liquid, purified, PBS, thimerosal.Also available as antiserum, PRB-101C)
-
Non-specific
immunogen = the twelve amino acid sequence CYPYDVPDYASL
recognizes the same hemagglutinin flu epitope YPYDVPDYA as the monoclonal antibody, HA.11 clone 16B12
Available from BioLegend
HA-probe (Y-11)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin.also available as: sc-805 AC;sc-805 HRP;;sc-805 FITC;sc-805 TRITC;biotin: sc-805 B;Alexa Fluor®ssc-805 AF405,sc-805AF488,sc-805 AF647and TransCruz reagent for ChIP, sc-805 X)
-
Non-specific
Immunogen = a peptide mapping within aninternal region of the influenza hemagglutinin (HA) protein
for detection of proteins containing theHA tag
-
GFP (3F8.2)
Chemicon and Upstate are Millipore
monoclonal
IgG1 κ
Mouse
(liquid, protein-G purified mouse immunoglobulin, PBS, azide)
-
Various vertebrate
immunogen = full length GFP protein
reacts with the 28 kDa GFP protein
-
Boitin
Anaspec
monoclonal
IgG1 ,κ
Mouse
(liquid, affintiy purified, PBS, BSA, azide)
-
Non-specific
Immunogen = asynthetic peptides with biotin conjugated to BSA
recognizes Bag-1
-
HA-tag (6E2)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, sodium HEPES, saline, BSA, glycerol, azideAvailable as Alexa Fluor® 488 Conjugate, #2350 and as Alexa Fluor® 647 Conjugate, #3444)
-
Non-specific
immunogen = synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA)
detects recombinant proteins containing the HA epitope tag; recognizes the HA-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells
-
FLAG (M2)
Sigma Aldrich
monoclonal
IgG1
Mouse
(purified Ig, buffered aqueous glycerol solution, TBS, glycerol, stabilizer, preservative, AP conj.also available as HRP conj., A8592 or biotin labeled, F9291)
-
Various species, Mouse, Rat
-
Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent; useful in detection of FLAG fusion proteins expressed in murine host
-
GFP Tag, IN
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Aequorea victoria
immunogen = a synthetic peptide corresponding to an internal sequence of green-fluorescent protein (GFP) isolated from Aequorea victoria
detects GFP
-
GFP Tag
Anaspec
polyclonal
IgY
Chicken
(liquid, affinity purified, PBS, azide)
-
Aequorea victoria
immunogen = full length GFP highly purified from Aequorea victoria
detects GFP
-
GFP Tag
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Aequorea victoria
immunogen = highly purified green-fluorescent protein (GFP) isolated directly from Aequorea victoria
detects GFP
-
GFP Tag, CT
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Aequorea victoria
immunogen = a synthetic peptide corresponding to the C-terminus of green-fluorescent protein (GFP) isolated from Aequorea victoria
detects GFP
-
GFP Tag, NT
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Aequorea victoria
immunogen = a synthetic peptide corresponding to the N-terminus of green-fluorescent protein (GFP) isolated from Aequorea victoria
detects GFP
-
FLAG (SIG1-25)
Sigma Aldrich
monoclonal
IgG1
Rabbit
(ascites fluid, azide)
-
Various species
immunogen = the FLAG™ peptide sequenceconjugated to KLH
reacts with N-terminaland C-terminal FLAG fusion proteins.
-
HIS-probe (H-15)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, purified, PBS, azide, gelatin.also available as: sc-803 AC for IP;sc-803 FITC for IF)
-
Non-specific
Immunogen = a peptide mapping to the polyhistidine domains of pET and Xpress polyhistidine expression vectors
for detection of fusion proteins encoded by prokaryotic pET, pRSET A, B, C and pTrc His A, B, C; baculovirus pBlue Bac His A, B, C and mammalian pEBV His A, B, C expression vectors
-
HIS-probe (G-18)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide, gelatin)
-
Non-specific
Immunogen = a peptide mapping ofHis-probe
for detection of fusion proteins encoded by pET expression vectors with polyhistidine sequence domains
-
HIS-probe (His17)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
-
Non-specific
Immunogen = a His-tagged recombinant protein
for detection of fusion proteins encoded by polyhistidine expression vectors
-
HIS-probe (H-3)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatinAvailable as biotin conjugate, sc-8036 B;as sc-8036 HRP, for WB;as sc-8036 AC, for IP)
-
Non-specific
Immunogen = a His-tagged recombinant protein
for detection of fusion proteins encoded by polyhistidine expression vectors
-
HIS-probe (HIS.H8)
Santa Cruz
monoclonal
IgG2b
Mouse
(liquid, PBS, azide, gelatin)
-
Non-specific
Immunogen = a 6x His-tagged polypeptide
for detection of fusion proteins encoded by polyhistidine expression vectors
-
HIS-probe (16-001)
Santa Cruz
monoclonal
IgG2b
Mouse
(liquid, PBS, azide, gelatin)
-
Non-specific
Immunogen = a 6x His-tagged homooligopeptide
for detection of linear HHH HHH origin
-
HIS-probe (16-002)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
-
Non-specific
Immunogen = a 6x His-tagged homooligopeptide
for detection of linear HHH HHH origin
-
HIS-probe (AD1.1.10)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
-
Non-specific
Immunogen = a polypeptide containing a 6X polyhistidine tag
for detection of fusion proteins encoded by polyhistidine expression vectors
-
GST Tag
Anaspec
monoclonal
IgG2a
Mouse
(liquid, PBS, BSA, azide)
-
Non-specific
immunogen = a synthetic GST peptide
detects GST
-
GST Tag
Anaspec
monoclonal
IgG2a
Mouse
(liquid, PBS, BSA, azide)
-
Non-specific
immunogen = a synthetic GST peptide
detects GST
-
GST Tag
Anaspec
monoclonal
IgG2a
Mouse
(liquid, PBS, BSA, azide)
-
Non-specific
immunogen = a synthetic GST peptide
detects GST
-
GST Tag
Anaspec
monoclonal
IgG2a
Mouse
(liquid, PBS, BSA, azide)
-
Non-specific
immunogen = a synthetic GST peptide
detects GST
-
GST Tag
Anaspec
monoclonal
IgG2a
Mouse
(liquid, PBS, BSA, azide)
-
Non-specific
immunogen = a synthetic GST peptide
detects GST
-